LawrenceGreenfieldMDPh.D., F.A.C.B.

PubMed

• Performance of chromogenic in situ hybridization on testing HER2 Status in breast carcinomas with chromosome 17 polysomy and equivocal (2+) herceptest results: a study...
  Gong, Y., Sweet, W., Duh, Y. J., Greenfield, L., Tarco, E., Trivedi, S., Symmans, W. F., Isola, J., Sneige, N.> ;Am J Clin Pathol. 2009 Aug
• Chromogenic in situ hybridization is a reliable method for detecting HER2 gene status in breast cancer: a multicenter study using conventional scoring criteria and the...
  Gong, Y., Sweet, W., Duh, Y. J., Greenfield, L., Fang, Y., Zhao, J., Tarco, E., Symmans, W. F., Isola, J., Sneige, N.> ;Am J Clin Pathol. 2009 Apr
• 40 citations
  DNA sequence of the araBAD promoter in Escherichia coli B/r.
  Lawrence Greenfield, Thomas C. Boone> ;Proceedings of the National Academy of Sciences of the United States of America. 1978 Oct 1
• 15 citations
  Hybrid plasmids containing the araBAD genes of Escherichia coli B/r.
  Donald A. Kaplan, Lawrence Greenfield, Thomas C. Boone> ;Gene. 1978 May 1
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Journal Articles

• Immunotoxin Therapy for Leptomeningeal Neoplasma.  
  Laske, D.W., Muraszko, K. M., Oldfield, E. H., DeVroom, H. L., Sung, C., Dedrick, R. L., Simon, T. R., Colandrea, J., Copeland, C., Katz, D., Greenfield, L., Groves, E..., Neurosurgery
• Reactivity of Murine Cytokine Fusion Toxin, Diphtheria Toxin390-Murine Interleukin-3 (DT390-mIL-3), With Bone Marrow Progenitor Cells.  
  Chan, C-H., Blazar, B.R., Greenfield, L., Kreitman R.J., and Vallera, D. A, Blood
• Rapid and Simple PCR Assay for Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma: Application to Acute Retroviral Infection.  
  Mulder, J., McKinney, N., Christopherson, C., Sninsky, J., Greenfield, L., and Kwok, S, J. Clin. Micro
• Pharmacokinetics and Toxicology of Immunotoxins Administered Into the Subarachnoid space in Nonhuman Primates and Rodents.  
  Muraszko,K., Sung,C., Walbridge, S., Greenfield, L., Dedrick, R.L., Oldfield, E.H., and Youle, R.J, Cancer Research
• Effect of Coupling Chemistry on the Pharmacology of Recombinant Ricin A Chain Immunotoxins.  
  Greenfield, L., and Dovey, H, Antibody, Immunoconjugates and Radiopharmaceuticals
• Thiol-Containing Cross-Linking Agent with Enhanced Steric Hindrance.  
  Greenfield, L., Bloch, W., and Moreland, M, Bioconjugate Chemistry
• A preliminary pharmacokinetic study of 111In-labeled 260F9 anti-(breast cancer) antibody in patients.  
  Griffin, T.W., Bokhari, F., Collins, J., Stochi, M., Bernier, M., Gionet, M., Siebecker, D., Wertheimer, M., Groves, E.S., Greenfield, L., Houston, L.L., Doherty, P.W...., Cancer Immunol. Immunother
• Improved immunotoxins engineered for increased tumor specificity: potential for treatment of central nervous system tumors and leptomeningeal neoplasia.  
  Johnson, V.G., Wrobel, C., Wilson, D., Zovickian, J., Greenfield, L., Oldfield, E.H., and Youle, R, J. of Neurosurgery
• The role of the diphtheria toxin receptor in cytosol translocation.  
  Johnson, V.G., Wilson, D., Greenfield, L., and Youle, R.J, Journal of Biological Chemistry
• Antibody-DT Conjugates in the Study of Surface Antigen Properties and DT Cytotoxic Mechanism.  
  Colombatti, M., Dell’Arciprete, L., Greenfield, L., Youle, R.J., Rappuoli, R., and Tridente, G, Bakteriol. Mikrobiol. Hyg. ABT
• Point Mutations in diphtheria toxin can separate binding from entry and amplify immunotoxin selectivity.  
  Greenfield, L., Johnson, V.G., and Youle, R.J, Science
• Functional expression of the human transferrin receptor cDNA in chinese hamster ovary cells deficient in endogenous transferrin receptor.  
  McGraw, T.E., Greenfield, L. and Maxfield, F.R, J. Cell Biology
• High level expression of diphtheria toxin peptides in Escherichia coli.  
  Greenfield, L., Dovey, H.F., Lawyer, F.C. and Gelfand, D.H, Bio/technology
• Cloned fragment of diphtheria toxin linked to T cell specific antibody identifies regions of B chain active in cell entry.  
  Colombatti, M., Greenfield, L. and Youle, R.J, J. Biol. Chem
• Nucleotide sequence of the structural gene for diphtheria toxin carried by corynebacteriophage beta.  
  Greenfield, L., Bjorn, M.J., Horn, G., Fong, D., Buck, G.A., Collier, R.J. and Kaplan, D.A, Proc. Natl. Acad. Sci
• Chromogenic method to screen very large populations of bacteriophage plaques for the presence of specific antigen.  
  Kaplan, D.A., Greenfield, L. and Collier, R.J, Methods in Enzymology
• The araC regulatory gene mRNA contains a leader sequence.  
  Cass, L., Horwitz, A., Miyado, G., Greenfield, L. and Wilcox, G, Molec. Gen. Genetics
• Conversion of closed circular DNA molecules to single nicked molecules by digestion with DNase I in the presence of ethidium bromide.  
  Greenfield, L., Simpson, L. and Kaplan, D, Biochim. Biophys. Acta
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Books/Book Chapters

• Sample Preparation Methods. in Diagnostic Molecular Microbiology: Principles and Applications. 
  Greenfield, L., and White, TEditors: Persing, D. H., Smith, T. F., Tenover, F. C., and White, T. J.American Society for Microbiology, Washington, D.C
• The Role of the CrossLinking Reagent in the Behavior Immunotoxins in Vitro and in Vivo. 
  Chapter: Therapeutic Proteins: Pharmacokinetics and Pharmacodynamics 
  Page 91 - 140Greenfield, LEditors: Kung, A. H. C., Baughman, R. A. and Larrick, J. W..Freeman and Company, New York: W. H
• Chemical and Genetic Characterization of the Enzymatic Activity Associated with Ricin A Chain. 
  Chapter: Genetically Engineered Toxins 
  Page 151 - 182Greenfield, LEditors: Frankel, A.Marcel Dekker, Inc, New York
• Genetics of Diphtheria Toxin Expression in Corynebacterium diphtheriae. 
  Chapter: Genetically Engineered Toxins 
  Page 273 - 305Greenfield, LEditors: Frankel, A.Marcel Dekker, Inc, New York
• Genetics of Diphtheria Toxin Production and Expression in Escherichia coli. 
  Chapter: Genetically Engineered Toxins 
  Page 307 - 338Greenfield, LEditors: Frankel, A.Marcel Dekker, Inc, New York
• Cancer Treatment and Research: Immunotoxins 
  Chapter: Genetic engineering of immunotoxins 
  Page 113 - 112Youle, R.J., Greenfield, L., and Johnson, V.GEditors: A.E. Franke.Martinus Nijhoff Publishing, Boston, Mass
• The appropriate toxicologic testing of recombinant human proteins. 
  Chapter: Banbury Report 29: Therapeutic Peptides and Proteins: Assessing the New Technologies 
  Page 175 - 187Marafino, B.J., Young, J.D., Greenfield, I.L., and Kopplin, J.Rcold spring harbor laboratory press
• Antibody toxin conjugates help clarify surface antigen behavior and toxin entry mechanism. 
  Chapter: Mediated Cytotoxicity and Cellular Biology, New Series 
  Page 115 - 165Colombatti, M., Greenfield, L., and Youle, R.JEditors: B. Bonavida, and R.J. Collier.Alan R. Liss, Inc, New York, N.Y
• Studies on the controlling site region of the araBAD operon of Escherichia coli B/r. 
  Chapter: Ribosomes and Nucleic Acid Metabolism 
  Page 287 - 299Wilcox, G., Greenfield, L. and Boone, TEditors: J. Zelinka and J. Balam.Publishing House of the Slovak Academy of Sciences
• Molecular cloning of segments of the M13 genome. 
  Chapter: The Single Stranded Bacteriophage 
  Page 461 - 466Kaplan, D.A., Greenfield, L. and Wilcox, GEditors: D. Denhardt, D. Dressler, and D. Ray.Cold Spring Harbor Laboratory Press
• Molecular cloning of the h80dara restriction fragments with noncomplementary ends. 
  Chapter: Molecular Approaches to Eucaryotic Genetic Systems 
  Page 85 - 102Kaplan, D.A., Greenfield, L. and Wilcox, GEditors: Wilcox, J. Abelson and C. F. Fox.Academic Press, New York
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Abstracts/Posters

• OncoScan™ FFPE Express 2.0: A Cancer-Focused Whole-Genome Copy Number Platform Requiring only 75-ng of Input DNA from FFPE Samples (OncoScan™ FFPE Services.
  Wang, Y., Sapolsky, R., Venkatapathy, S., Siddiqui, F., Shen, F., Thompson, P., Campbell, I., Stengel, G., Estabrook, A., Shukla, A., Weaver, P., Wai, W., Huynh, V., a..., AACR 2012 Annual Meeting, Chicago, Illinois
• Chromogenic In Situ Hybridization (CISH) Is A Reliable Method For Detecting HER-2 Gene Status in Breast Cancer: A Multicenter Study Comparing Three Methods.
  Gong, Y., Sweet, W., Duh, Y.J., Greenfield, L., Fang, Y., Zhao, J., Tarco, E., Symmans, W.F., Isola, J., and Sneige, N, USCAP Annual Meeting, Denver, Colorado
• Impact of Chromosome 17/HER-2 Polysomy on Reliability of Chromogenic In Situ Hybridization (CISH): A Study of Two Institutions.
  Gong, Y., Sweet, W., Duh, Y.J., Greenfield, L., Symmans, W.F., Isola, J., and Sneige, N, USCAP Annual Meeting, Denver, Colorado
• Effect of Immunohistochemistry (IHC)-Equivocal Breast Carcinoma On Performance of Chromogenic In Situ Hybridization (CISH) and Inter-Site Reproducibility.
  Gong, Y., Sweet, W., Duh, Y.J., Greenfield, L., Trivedi, S., Symmans, W.F., Isola, J., and Sneige, N, USCAP Annual Meeting, Denver, Colorado
• Indirect Labeling of Antibodies Targeted Towards Tumors For Small Animal In Vivo Imaging.
  Huang Z., Aggeler B., Chen G., Greenfield L., Lee F., Nyhus J., Shallice M., Wickramsinghe D., Wu F, and Leong L, Imaging in 2020: A Conference on Molecular Imaging, Jackson Hole, Wyoming
• Functional Labeling of In Vivo Imaging Probes.
  Steinberg, T.H., Nyhus, J.K., Aggler, B., Leong, L., and Greenfield, L, Fifth Annual Meeting of The Society for Molecular Imaging, Hawaii
• Vascular Contrast Agents for Optical In Vivo Imaging.
  Nyhus, J.K., Steinberg, T.H., Leong, L., and Greenfield, L, Fifth Annual Meeting of The Society for Molecular Imaging, Hawaii
• Optical Imaging Probes for In Vivo Tumor Biology.
  Steinberg, T.H., Nyhus, J.K., Aggler, B., Greenfield, L., and Leong, L, Cambridge Healthtech’s Third Annual In Vivo Molecular Imaging, San Diego, CA
• Multiplex In vivo Imaging: Imaging Multiple Targets To Gain Spatial And Temporal Context Of the In Vivo Events.
  Greenfield, L, Symposium for In Vivo Small Animal Imaging, Northwestern University. Chicago, IL
• Optical Diagnostic Imaging From Bench to Bedside at the National Institute of Health.
  Greenfield, L, Optical Imaging 2006 Fifth Inter-Institute Workshop, Bethesda, Maryland
• Fluo-4 NW: A New No Wash Calcium Assay for HTS.
  Dzubay, J., Schulenberg, B., Martin, V., Antoulinakis, E., Berlier, J., Hoffman, R., Greenfield, L., Johnson, I, Drug Discovery Technology and Development, Boston, MA
• Automated Nucleic Acid Sample Preparation For Genomic Research.
  Montesclaros, L., Felton, A., Voice, R., Somack, R., Gentile, A., Bi, W., Borodkin, M., Hosizaki, J., Livak, K., Luo, Q., Mirchandani, V., Sandell, D., Sandell, S., Th..., Genomic Sample Preparation Conference, Boston, Massachusetts
• Automated Total RNA Extraction From Whole Blood Using The ABI Prism 6700 Workstation.
  Tanner, M., Felton, A., Voice, R., Greenfield, L., Ruff, D., and Snider, J, Genomic Sample Preparation Conference, Boston, Massachusetts
• Automated Total RNA Extraction From Whole Blood Using The ABI Prism 6700 Workstation.
  Tanner, M., Felton, A., Voice, R., Greenfield, L., Ruff, D., and Snider, J, 92nd Annual Meeting of the American Association for Cancer Research, New Orleans, Louisiana
• Automated Nucleic Acid Sample Preparation For Genomics Research.
  Montesclaros, L., Felton, A., Voice, R., Somack, R., Gentile, A., Bi, W., Borodkin, M., Hosizaki, J., Livak, K., Luo, Q., Mirchandani, V., Reed, M., Sandell, D., Sande..., Lab Automation, Palm Springs, California
• Methods for Rendering Mycobacteria in Clinical Specimens Non-Viable.
  Young, K., Mohr, K., Dodge, D., Greenfield, L., and Koenig, G, American Society for Microbiology
• Quantitation of HIV-1 Plasma Viremia by PCR with Tth DNA Polymerase.
  Christopherson, C., Mulder, J., McKinney, N., Greenfield, L., Schwartz, R., Russell, M., Spahlinger, T., Jackson, J. B., and Kwok, ICAAC
• A PCR-based Assay That Utilizes the Inherent 5' of rTth DNA Polymerase for the Quantitative Detection of HCV Detection of HCV RNA.
  Tsang, S.Y., Kwok, S., and Greenfield, L, ASM Conference on Molecular Diagnostics and Therapeutics, Moran, Wyo
• A Simple Method for the Detection and Quantitation of Low Amounts of a Specific DNA Target Utilizing the Polymerase Chain Reaction.
  Greenfield, L., Groetsema, G., Watson, R., Holland, P. M., Will, S., and Bloch, W, 203rd American Chemical Society National Meeting, San Francisco
• Modifications of Diphtheria toxin for use in immunotoxins.
  Greenfield, L., Johnson, V.J., Colombatti, M., and Youle, R, Society for Industrial Microbiology, Chicago, Illinois
• Pharmacokinetics and efficacy of immunotoxins containing recombinant ricin A chain.
  Greenfield, L., Bell, D., Apell, G., and Young, J, International Symposium on immunotoxins, Duke University, Durham, North Carolina
• Point mutations in diphtheria toxin separate binding from entry and amplify immunotoxin selectivity.
  Johnson, V.G., Greenfield, L., Wilson, D., Wrobel, C., and Youle, R, presented at the 3rd International Conference on monoclonal antibody immunoconjugates for cancer, San Diego, California
• Point mutations in diphtheria toxin separate binding from entry.
  Johnson, V.G., Greenfield, L., Wilson, D.J., and Youle, R.J, 27th Annual meeting of the American Society for Cell Biology, S. Louis, Missouri
• Engineering of diphtheria toxin for use in immunoconjugates.
  Greenfield, L. and Dovey, R, J. of Cellular Biochemistry
• Expression of the human transferrin receptor in a chinese hamster ovary cell line deficient in endogenous transferrin receptor.
  McGraw, T.E., Greenfield, L. and Maxfield, F.R, J. of Cell Biology
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Other

• Methods And Kits For Obtaining Nucleic Acid From Biological Samples. 
  Montesclaros, L. and Greenfield, L, Patent Application Number WO2005007895
• Methods For Selecting Protein Binding Moieties. 
  Greenfield, I. L, Patent Application Number WO2005001130
• Fluorescent Polarization Assay. 
  Sekar, M. M. and Greenfield, I. L, Patent Application Number WO2004013628
• Method For PCR Cleanup and Oligonucleotide Removal. 
  Greenfield, L., and Bost, D.A, Patent Number WO 2004/009851 A2
• Heteroconfigurational Polynucleotide And Methods Of Use. 
  Greenfield, I.L., Matysiak, S.M., Schroeder, B.V., and Vinayak, R, Patent Number WO 03/059929 A1
• Compositions, Methods, And Kits For Isolating Nucleic Acids Using Surfactants And Proteases. 
  Bost, D.A., and Greenfield, L, Patent Number WO 02/090539 A2
• ) Coupling Agents and Sterically Hindered Disulfide Linked Conjugates Prepared Therefrom. 
  Moreland, M., Greenfield, I. L., and Niteck, D. E, Patent Number EP428534B1
• Purified Thermostable Nucleic Acid Polymerase Enzyme From Thermosiho africanus. 
  Gelfand, D. H., Lawyer, F. C., Abramson, R. D., Greenfield, I. L., Reichert, F. L, Patent Number: WO9206202A1
• Toxin Conjugates. 
  Greenfield, L. I., Nitecki, D. E., Kaplan, D. A., Gelfand, D. H., Piatak, M., Horn, G., Lawyer, F. C, Number: EP170697B1
• oxin Conjugates. 
  Greenfield, L. I., Nitecki, D. E., Kaplan, D. A., Gelfand, D. H., Piatak, M., Horn, G, Patent Number: WO8503508A1
• Coupling Agents And Sterically Hindered Disulfide Linked Conjugates Prepared Therefrom. 
  Moreland, M., Greenfield, I. L., Nitecki, D, Patent Number: WO8912624
• Recombinant Methods for the Production of Ricin A, Ricin B, Ricin or Diphtheria Toxin (DT)A or Ab’ Fragment, Suitable Hosts And Vectors Therefor. 
  Gelfand, D., Lawyer, F. C., Horn, G., Greenfield, L., Nitecki, D., Kaplan, D., Piatak, M. Jr, Patent Number: EP335476A3
• Composition Method and Kit for Reducing Background Staining. 
  Filanoski, B., Greenfield, I.L. and Hirsch, J.D, Patent Application US 20080038772
• Compositions and Methods of Selective Nucleic Acid Isolation. 
  Bost, D. and Greenfield, L, Patent Application: US 20070224630
• Control of Chemical Modification. 
  Mauro, J.M., Steinberg, T.H.; Greenfield, L.I., and Leong, L, Patent Application US 20070134685
• Fluorescent Polarization Assay. 
  Sekar, M. M. A. and Greenfield, I.L, Patent Application US 20070054308
• Heteroconfigurational Polynucleotides and Methods of Use. 
  Greenfield, I. L., Matysiak, S.M., Schroeder, B.G., and Vinayak, R. S, Patent Application US 20060292438 A1
• Heteroconfigurational Polynucleotides and Methods of Use. 
  Matysiak, S.M.; Schroeder, B.G., Vinayak, R.S., and Greenfield, I.L, Patent Application US 20060286581
• Optically-Detectable Enzyme Substrates and Their Method Of Use. 
  Corry, S.B., Downey, W.L., Filanoski, B., Gee, K.R., Greenfield, I.L., Hirsch, J.D., Johnson, I., and Rukavishnikov, A, Patent Application US 20050227309
• Multiplex Protease Profiling. 
  Sun, H., Greenfield, L., and Bost, D.A, Patent Application 20050176076
• Methods and Kits For Obtaining Nucleic Acid From Biological Samples. 
  Montesclaros, L. and Greenfield, L, US 20050009036
• Methods For Selecting Protein Binding Moieties. Patent Application. 
  Greenfield, L, US 20040248109
• Method For Generating Multiple Samples Containing A Predetermined Amount of Nucleic Acid. 
  Greenfield, I. L., and Bost, D.A, Patent Application. US 20040121136
• Heteroconfigurational Polynucleotides And Methods Of Use. 
  Greenfield, L., Matysiak, Stefan M., Schroeder, B., Vinayak, R, Patent Application Number 20030198980
• Optically-Detectable Enzyme Substrates and Their Method of Use. 
  Corry, S., Downey, W., Filanoski, B. Gee, K. Greenfield, I.L., Hirsch, J., Johnson, I., and Rukavishnikov, A, Patent Application Number W02005071096
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